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This work has been funded by grant PID2019-106222RB-C31 funded by MCIN. Jorge Rojas Lopez-Menchero has been supported by a predoctoral contract associated to the project PID2019-106222RB-C31 funded by MCIN/AEI/https://doi.org/10.13039/501100011033 and FSE "El FSE invierte en tu futuro".

Analysis of institutional authors

Lopez-Menchero, Jorge RojasAuthor

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Article

Advanced fermentation techniques enhance dioxolanone type biopesticide production from Phyllosticta capitalensis

Publicated to:Scientific Reports. 15 (1): 7989- - 2025-03-07 15(1), DOI: 10.1038/s41598-025-92626-0

Authors: Castillo, Nicolas Reyes; Lopez-Menchero, Jorge Rojas; Useche, William A Pacheco; Diaz, Carmen E; Andres, M Fe; Gonzalez-Coloma, Azucena

Affiliations

CSIC, Inst Ciencias Agr ICA, Serrano 115Dpdo, Madrid 28006, Spain - Author
Inst Prod Nat & Agrobiol IPNA, CSIC, Avda Astrofisico F Sanchez 3, San Cristobal la Laguna 38206, Tenerife, Spain - Author
Justus Liebig Univ Giessen, Inst Insect Biotechnol Focus Nat Prod Res, Ohlebergsweg 12, D-35392 Giessen, Germany - Author
Univ Politecn Madrid UPM, Dept Biotecnol Biol Vegetal, Escuela Tecn Super Ingn Agr Alimentaria & Biosiste, Madrid 28040, Spain - Author

Abstract

In the current work, the production of dioxolanone and meroterpene type secondary metabolites under different fermentation conditions by the endophytic fungus Phyllosticta capitalensis (isolate YCC4) isolated from the leaves of the endemic plant Persea indica has been evaluated. Different techniques, such as microparticle-enhanced culture (MPEC: bentonite, talcum powder) and surface adhesion fermentation (SAF: metallic mesh and glass wool) were applied to the culture medium. Ethyl acetate extracts from different fermentations of the isolate YCC4 were analyzed for their content and revealed the following dioxolanone derivatives: metguignardic acid (1), guignardianone C (2), ethyl guignardate (3), guignardianone D (4) and phenguignardic acid methyl ester (5), and meroterpenes: guignardone A (6) and B (7), guignarenone C (8), guignarenone B (9) and guignardone I (10). Additionally, the biological activity of extracts was tested against the aphid Myzus persicae and the root-knot nematode Meloidogyne javanica. Our study revealed significant variations in the production of the target metabolites as well as notable differences in biopesticide activity influenced by the presence of inert supports. Overall, the findings indicate that glass wool (GW) is a high-performance material to improve the production of dioxolanone derivatives. These findings underscore the significance of innovative cultivation methods such MPEC and SAF as drivers to produce valuable secondary metabolites from fungal organisms.

Keywords

AciAnimalsAphidsAscomycotaAspergillus-nigerBentoniteBiological control agentsBiopesticidesDioxolanoneDioxolanonesFermentationFungal endophyteMicroparticle-enhanced culture (mpec)Plant leavesRhizopus-oryzaeSolid-stateSurface adhesion fermentation (saf)

Quality index

Bibliometric impact. Analysis of the contribution and dissemination channel

The work has been published in the journal Scientific Reports due to its progression and the good impact it has achieved in recent years, according to the agency WoS (JCR), it has become a reference in its field. In the year of publication of the work, 2025, it was in position 25/134, thus managing to position itself as a Q1 (Primer Cuartil), in the category Multidisciplinary Sciences.

Impact and social visibility

From the perspective of influence or social adoption, and based on metrics associated with mentions and interactions provided by agencies specializing in calculating the so-called "Alternative or Social Metrics," we can highlight as of 2025-06-29:

  • The use of this contribution in bookmarks, code forks, additions to favorite lists for recurrent reading, as well as general views, indicates that someone is using the publication as a basis for their current work. This may be a notable indicator of future more formal and academic citations. This claim is supported by the result of the "Capture" indicator, which yields a total of: 6 (PlumX).

It is essential to present evidence supporting full alignment with institutional principles and guidelines on Open Science and the Conservation and Dissemination of Intellectual Heritage. A clear example of this is:

  • The work has been submitted to a journal whose editorial policy allows open Open Access publication.

Leadership analysis of institutional authors

This work has been carried out with international collaboration, specifically with researchers from: Germany.