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Perez-Alonso, MmAuthorOrtiz-Garcia, PAuthorMoya-Cuevas, JAuthorSanchez-Parra, BAuthorWilkinson, MdAuthorPollmann, SCorresponding Author

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May 31, 2021
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Article

Endogenous indole-3-acetamide levels contribute to the crosstalk between auxin and abscisic acid, and trigger plant stress responses in Arabidopsis

Publicated to: Journal Of Experimental Botany. 72 (2): 459-475 - 2021-02-02 72(2), DOI: 10.1093/jxb/eraa485

Authors: Perez-Alonso, Marta-Marina; Ortiz-Garcia, Paloma; Moya-Cuevas, Jose; Lehmann, Thomas; Sanchez-Parra, Beatriz; Bjork, Robert G.; Karim, Sazzad; Amirjani, Mohammad R.; Aronsson, Henrik; Wilkinson, Mark D.; Pollmann, Stephan;

Affiliations

‎ Arak Univ, Dept Biol, Arak, Iran - Author
‎ Gothenburg Global Biodivers Ctr, S-40530 Gothenburg, Sweden - Author
‎ Max Planck Inst Chem, Mainz, Germany - Author
‎ Ruhr Univ Bochum, Lehrstuhl Pflanzenphysiol, D-44801 Bochum, Germany - Author
‎ Univ Gothenburg, Dept Biol & Environm Sci, S-40530 Gothenburg, Sweden - Author
‎ Univ Gothenburg, Dept Earth Sci, S-40530 Gothenburg, Sweden - Author
‎ Univ Gothenburg, Sahlgrenska Acad, Inst Biomed, Dept Infect Dis, S-40530 Gothenburg, Sweden - Author
‎ Univ Politecn Madrid UPM, Dept Biotecnol Biol Vegetal, Escuela Tecn Super Ingn Agron Alimentaria & Biosi, Madrid 28040, Spain - Author
‎ Univ Politecn Madrid UPM, Inst Nacl Invest & Tecnol Agr & Alimentaria INIA, Ctr Biotecnol & Genom Plantes, Pozuelo De Alarcon 28223, Spain - Author
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Abstract

The evolutionary success of plants relies to a large extent on their extraordinary ability to adapt to changes in their environment. These adaptations require that plants balance their growth with their stress responses. Plant hormones are crucial mediators orchestrating the underlying adaptive processes. However, whether and how the growth-related hormone auxin and the stress-related hormones jasmonic acid, salicylic acid, and abscisic acid (ABA) are coordinated remains largely elusive. Here, we analyse the physiological role of AMIDASE 1 (AMI1) in Arabidopsis plant growth and its possible connection to plant adaptations to abiotic stresses. AMI1 contributes to cellular auxin homeostasis by catalysing the conversion of indole-acetamide into the major plant auxin indole-3-acetic acid. Functional impairment of AMI1 increases the plant's stress status rendering mutant plants more susceptible to abiotic stresses. Transcriptomic analysis of ami1 mutants disclosed the reprogramming of a considerable number of stress-related genes, including jasmonic acid and ABA biosynthesis genes. The ami1 mutants exhibit only moderately repressed growth but an enhanced ABA accumulation, which suggests a role for AMI1 in the crosstalk between auxin and ABA. Altogether, our results suggest that AMI1 is involved in coordinating the trade-off between plant growth and stress responses, balancing auxin and ABA homeostasis.

Keywords

Abiotic stressAbscisic acidArabidopsisArabidopsis proteinsArabidopsis thalianaAuxin biosynthesisBiosynthesisExpressionGeneGene expression regulation, plantGrowthHomeostasisHormoneHydrolaseIndole-3-acetamideIndole-3-acetic acidIndoleacetic acidsMediated regulationMetabolismPlant developmentPlant growth regulatorsPlant hormone crosstalkTranscription factors

Quality index

Bibliometric impact. Analysis of the contribution and dissemination channel

The work has been published in the journal Journal Of Experimental Botany due to its progression and the good impact it has achieved in recent years, according to the agency WoS (JCR), it has become a reference in its field. In the year of publication of the work, 2021, it was in position 15/239, thus managing to position itself as a Q1 (Primer Cuartil), in the category Plant Sciences. Notably, the journal is positioned above the 90th percentile.

From a relative perspective, and based on the normalized impact indicator calculated from World Citations provided by WoS (ESI, Clarivate), it yields a value for the citation normalization relative to the expected citation rate of: 1.02. This indicates that, compared to works in the same discipline and in the same year of publication, it ranks as a work cited above average. (source consulted: ESI Nov 13, 2025)

This information is reinforced by other indicators of the same type, which, although dynamic over time and dependent on the set of average global citations at the time of their calculation, consistently position the work at some point among the top 50% most cited in its field:

  • Weighted Average of Normalized Impact by the Scopus agency: 2.77 (source consulted: FECYT Mar 2025)

Specifically, and according to different indexing agencies, this work has accumulated citations as of 2025-12-05, the following number of citations:

  • WoS: 14
  • Scopus: 38
  • Europe PMC: 3
  • Google Scholar: 33

Impact and social visibility

From the perspective of influence or social adoption, and based on metrics associated with mentions and interactions provided by agencies specializing in calculating the so-called "Alternative or Social Metrics," we can highlight as of 2025-12-05:

  • The use, from an academic perspective evidenced by the Altmetric agency indicator referring to aggregations made by the personal bibliographic manager Mendeley, gives us a total of: 54.
  • The use of this contribution in bookmarks, code forks, additions to favorite lists for recurrent reading, as well as general views, indicates that someone is using the publication as a basis for their current work. This may be a notable indicator of future more formal and academic citations. This claim is supported by the result of the "Capture" indicator, which yields a total of: 53 (PlumX).

With a more dissemination-oriented intent and targeting more general audiences, we can observe other more global scores such as:

  • The Total Score from Altmetric: 3.
  • The number of mentions on the social network X (formerly Twitter): 4 (Altmetric).

It is essential to present evidence supporting full alignment with institutional principles and guidelines on Open Science and the Conservation and Dissemination of Intellectual Heritage. A clear example of this is:

  • The work has been submitted to a journal whose editorial policy allows open Open Access publication.

Leadership analysis of institutional authors

This work has been carried out with international collaboration, specifically with researchers from: Germany; Iran; Sweden.

There is a significant leadership presence as some of the institution’s authors appear as the first or last signer, detailed as follows: First Author (Perez Alonso, Marta Marina) and Last Author (POLLMANN, STEPHAN).

the author responsible for correspondence tasks has been POLLMANN, STEPHAN.

Awards linked to the item

The authors thank the NASC for providing seeds of T-DNA insertion and mutant lines, and Prof. Nam-Hai Chua (The Rockefeller University, New York) for providing the pMDC7 vector. Moreover, SP is grateful to Prof. Elmar W. Weiler (Ruhr-Universitat Bochum, Germany) for his support in the initial phase of the AMI1 project. This research was supported by grants from the German Research Foundation (DFG, SFB480/A10) and the Spanish Ministry of Economy, Industry and Competitiveness (MINECO, BFU201782826-R to SP and a grant from the Swedish Research Council (VR) to HA. JM was supported by the `Severo Ochoa Program for Centers of Excellence in R&D' from the Agencia Estatal de Investigacion of Spain, grant SEV-2016-0672 (2017-2021) to the CBGP.